Bone Morphogenetic Proteins (BMPs) are part of the TGF-β superfamily, a group of structurally related signaling proteins. Among them, BMP-2 stands out as a potent osteoinductive cytokine, capable of prompting bone and cartilage formation when coupled with osteoconductive carriers like collagen and synthetic hydroxyapatite. Besides its role in osteogenesis, BMP-2 contributes significantly to cardiac morphogenesis and is expressed in various tissues such as the lung, spleen, brain, liver, prostate, ovary, and small intestine.
The active form of BMP-2 is a 26 kDa protein made up of two identical 114 amino acid polypeptide chains connected by a single disulfide bond. Each BMP-2 monomer is part of a precursor polypeptide, featuring a 23 amino acid signal sequence for secretion and a 259 amino acid propeptide. Upon precursor dimerization, a furin-type protease cleaves the covalent bonds between the propeptide (also a disulfide-linked homodimer) and the mature BMP-2 ligand.
Recombinant Human/Murine/Rat BMP-2 is a 26.0 kDa homodimeric protein composed of two 115 amino acid polypeptide chains.
|
Product Specifications
|
|
| Species |
Human, Mouse, Rat
|
| Published species |
Human, Mouse
|
| Expression System | E. coli |
| Amino acid sequence |
MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR
|
| Molecular weight | 26 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity |
≥ 98% by SDS-PAGE gel and HPLC analyses.
|
| Endotoxin concentration | <0.1 EU/µg |
| Activity |
Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1.0 ug/ml.
|
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification |
HPLC, SDS-PAGE
|
| Contains |
no preservative
|
| Storage conditions | -20°C |
CAUTION
For Research Use Only. Not for use in diagnostic procedures.
