Flt3-Ligand is a growth factor responsible for regulating the proliferation of early hematopoietic cells. It binds to cells expressing the Flt3 tyrosine kinase receptor. Interestingly, Flt3-Ligand itself doesn't directly stimulate the proliferation of early hematopoietic cells. Instead, it works synergistically with other colony-stimulating factors (CSFs) and interleukins to induce growth and differentiation. Unlike SCF, Flt3-Ligand doesn't affect mast cells.
Various isoforms of Flt3-Ligand have been identified, with the predominant biologically active form being anchored to the cell surface as part of a transmembrane protein (209 amino acids). This membrane-bound isoform can undergo proteolytic cleavage, producing a biologically active soluble isoform.
Recombinant Human Flt3-Ligand is a soluble protein weighing 17.6 kDa and composed of 155 amino acid residues.
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Product Specifications
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| Species | Human |
| Published species |
Hamster, Human, Mouse, Rabbit
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| Expression System | E. coli |
| Amino acid sequence |
TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA
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| Molecular weight | 17.6 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity |
≥ 98% by SDS-PAGE gel and HPLC analyses.
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| Endotoxin concentration | <0.1 EU/µg |
| Activity |
The ED50 as determined by the dose-dependent stimulation of the proliferation of human AML5 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10^6 units/mg.
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| Conjugate | Unconjugated |
| Form | Lyophilized |
| Contains |
no preservative
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| Storage conditions | -20°C |
CAUTION
For Research Use Only. Not for use in diagnostic procedures.
