IL-10, an immunosuppressive cytokine, is synthesized by various mammalian cell types like macrophages, monocytes, T cells, B cells, and keratinocytes. Its primary function is to dampen the expression of proinflammatory cytokines such as IL-1 and TNF-α. Similar to IL-4, IL-10 boosts humoral immune responses while tempering cell-mediated immune reactions.
Interestingly, Human IL-10 exhibits activity on murine cells, but the reverse is not true—murine IL-10 doesn't affect Human cells. The recombinant Human IL-10, a protein weighing 18.6 kDa and comprising 161 amino acid residues, reflects these immunomodulatory properties.
|
Product Specifications
|
|
| Species | Human |
| Published species | Human, Virus |
| Expression System | E. coli |
| Amino acid sequence |
MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N
|
| Molecular weight | 18.6 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity |
≥ 98% by SDS-PAGE gel and HPLC analyses.
|
| Endotoxin concentration | <0.1 EU/µg |
| Activity |
Determined by the dose-dependent co-stimulation (with human IL-4) of MC/9 cells. The expected ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 10^5 units/mg.
|
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Contains |
no preservative
|
| Storage conditions | -20°C |
CAUTION
For Research Use Only. Not for use in diagnostic procedures.
