Leukemia Inhibitory Factor (LIF) is a ~20 kDa cytokine in the IL-6 family. It signals through the LIF receptor and gp130. LIF is found in tissues such as the lungs, thymus, and nervous system and can increase during inflammation or tissue injury. It has many biological effects, but is best known for supporting stem cell self-renewal and preventing embryonic stem cell differentiation, particularly in mice. Human and mouse LIF are about 79% similar and can show cross-species activity.
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Product Specifications
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| Species | Human |
| Published species |
Human, Rat
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| Expression System | E. coli |
| Amino acid sequence | SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF |
| Molecular weight | 19.6 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity |
≥ 98% by SDS-PAGE gel and HPLC analyses.
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| Endotoxin concentration | <0.1 EU/µg |
| Activity | Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.075 ng/ml, corresponding to a specific activity of ≥ 1.33 x 10^7 units/mg. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Contains |
no preservative
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| Storage conditions | -20°C |
CAUTION
For Research Use Only. Not for use in diagnostic procedures.
