M-CSF, a potent hematopoietic factor, is generated by a variety of cells such as lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts, and osteoblasts. It serves as a crucial regulator of cellular proliferation, differentiation, and survival for blood monocytes, tissue macrophages, and their progenitor cells. Notably, M-CSF plays significant roles in modulating dermal thickness and fertility. Clinically, it finds use in treating infections, malignancies, and atherosclerosis, aiding hematopoietic recovery post bone marrow transplantation. While Human M-CSF is reactive in murine systems, the murine molecule lacks activity on Human cells. Recombinant Human M-CSF is a homodimeric protein weighing 36.8 kDa, composed of two 159 amino acid polypeptide subunits.
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Product Specifications
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| Species | Human |
| Published species |
Human, Mouse, Rat
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| Expression System | E. coli |
| Amino acid sequence |
MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS
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| Molecular weight | 36.8 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity |
≥ 98% by SDS-PAGE gel and HPLC analyses.
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| Endotoxin concentration | <0.1 EU/µg |
| Activity |
The ED50 was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10^6 units/mg.
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| Conjugate | Unconjugated |
| Form | Lyophilized |
| Contains |
no preservative
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| Storage conditions | -20°C |
CAUTION
For Research Use Only. Not for use in diagnostic procedures.
