VEGF stands out as a potent cytokine, driving growth and angiogenesis. It fosters endothelial cell proliferation and survival, contributing significantly to angiogenesis and vascular permeability. Its expression in vascularized tissues plays a crucial role in both normal and abnormal angiogenesis. VEGF's involvement is notable in tumor metastasis induction and intraocular neovascular syndromes.
VEGF signals through three receptors: fms-like tyrosine kinase (flt-1), the KDR gene product (with the murine equivalent being the flk-1 gene product), and the flt4 gene product. Recombinant Human VEGF165 is a 38.2 kDa, disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.
Product Specifications | |
Species | Human |
Published species |
Bovine, Chicken, Dog, Fish, Hamster, Horse, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Sheep
|
Expression System | E. coli |
Amino acid sequence |
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
|
Molecular weight | 38.2 kDa |
Class | Recombinant |
Type | Protein |
Purity |
≥ 98% by SDS-PAGE gel and HPLC analyses.
|
Endotoxin concentration | <1 EU/µg |
Activity |
Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-8.0 ng/ml.
|
Conjugate |
Unconjugated
|
Form | Lyophilized |
Contains |
no preservative
|
Storage | -20°C
|
CAUTION
For Research Use Only. Not for use in diagnostic procedures.